细胞名称 | BEAS-2B 人正常肺上皮细胞 |
描述 | 从一位非癌个体的正常人支气管上皮病理切片分离出上皮细胞。 这些细胞用腺病毒12-SV40病毒杂交病毒感染并克隆。 DEAS-2B细胞保留了对血清反应进行鳞关分化的能力，并有用于筛选诱导或影响分化及致癌的化学或生物制剂。 细胞角蛋白及SV40抗原染色阳性。 |
动物种别 | 人 |
性别 | 未知 |
组织来源 | 肺;支气管;正常;上皮;病毒转化 |
形态 | 上皮细胞样 |
培养基和添加剂 | 订购此细胞需提供100ml培养基。LHC-9培养液添加0.5钠克/毫升重组上皮细胞生长因子(EGF),500钠克/毫升氢化可的松，0.035钠克/毫升牛垂体提取物，500mM乙醇胺，500nM 磷酸乙醇胺，0.01毫克/毫升肾上腺素，0.1钠克/毫升retinoic酸及微量元素。注意：不要过滤EGF。LHC-9基础培养液可以从Biofluids, Inc., Rockville, MD(目录号118)或Clonetics Corporation, Walkersville, MD 21793(BEBM目录 |
供应限制 | A。仅供研究之用。 |
REFERENCE | Reddel RR , et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 Lechner JF , LaVeck MA . A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985. Sakamoto O , et al. Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. J. Clin. Invest. 99: 701-709, 1997. PubMed: 9045873 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
中文名称 | 人正常肺上皮细胞 |
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货号： BEAS-2B 基本售价： 询价 规格： -