细胞名称

MCF7 [MCF-7]人乳腺癌细胞

描述

MCF-7细胞保留了多个分化了的乳腺上皮的特性，包括：能通过胞质雌激素受体加工雌二醇并能形成圆形复合物（domes）。 该细胞含有Tx-4癌基因。 肿瘤坏死因子α（TNF alpha）可以抑制MCF-7细胞的生长。 抗雌激素处理细胞能调变IGFBP’S的分泌。

动物种别

人

性别

女

组织来源

腺癌，乳腺，胸水。

形态

上皮细胞

培养基和添加剂

MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L，0.01mg/ml bovine insulin)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

Sugarman BJ , et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Takahashi K , Suzuki K . Association of insulin-like growth-factor-I-induced DNA synthesis with phosphorylation and nuclear exclusion of p53 in human breast cancer MCF-7 cells. Int. J. Cancer 55: 453-458, 1993. PubMed: 8375929 Brandes LJ , Hermonat MW . Receptor status and subsequent sensitivity of subclones of MCF-7 human breast cancer cells surviving exposure to diethylstilbestrol. Cancer Res. 43: 2831-2835, 1983. PubMed: 6850594 Lan MS , et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 Pratt SE , Pollak MN . Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993. PubMed: 7693333 Huguet EL , et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 Soule HD , et al. A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51: 1409-1416, 1973. PubMed: 4357757 Bellet D , et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 Komarova EA , et al. Intracellular localization of p53 tumor suppressor protein in gamma-irradiated cells is cell cycle regulated and determined by the nucleus. Cancer Res. 57: 5217-5220, 1997. PubMed: 9393737 van Dijk MA , et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997. PubMed: 9270016 Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Umekita Y , et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218 Zamora-Leon SP , et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847 Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chang K , Pastan I . Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224 Bacus SS , et al. Differentiation of cultured human breast cancer cells (AU-565 and MCF-7) associated with loss of cell surface HER-2/neu antigen. Mol. Carcinog. 3: 350-362, 1990. PubMed: 1980588 Acta Biochimica et Biophysica Sinica 34(2):165-170,2002 Chin J Oncol. 24(4):327-330,2002 Jiangsu Med J. 28(9):641-643,2002 Zhejiang Med 24(10):586-588,2002 Chin J Oncol. 25(4):332-334,2003 Chinese Journal of Cancer 22(3):257-261,2003 Chinese Journal of Cancer 22(4):380-384,2003 The Practical Journal of Cancer 18(5):457-459,2003 Research in Tumor Prev Treat 30(3):210-213,2003 China J Cancer Prev Treat 10(6):583-585,2003 J First Mil Med Univ 23(7):731-733,2003 Acta Academae Medicinae Miltaris Tertiae 25(2):151-154,2003 Acta Universitatis Medicinalis Nanjing (Natural Science) 23(2):134-137,2003