细胞名称 | HEp-2人喉表皮样癌细胞 |
描述 | 最初认为这个细胞源自喉上皮癌,但随后通过同功酶分析、HeLa标记染色体和DNA指纹分析发现,起源细胞已被HeLa污染。 角蛋白免疫过氧化物酶染色阳性。 |
动物种别 | 人 |
性别 | *** |
组织来源 | HeLa 污染 |
形态 | 上皮细胞 |
培养基和添加剂 | RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),80%;胎牛血清,20%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 |
供应限制 | A。仅供研究之用。 |
REFERENCE | Moore AE , et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res. 15: 598-602, 1955. PubMed: 13261081 Chen TR . Re-evaluation of HeLa, HeLa S3, and Hep-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844 Toolan HW . Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3; and H.Emb.Rh. No. 1. Cancer Res. 14: 660-666, 1954. PubMed: 13209540 Black FL , et al. Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2). Proc. Soc. Exp. Biol. Med. 93: 107-108, 1956. PubMed: 13370591 Tex. Rep. Biol. Med. 15: 588, 1957. Moore AE . Tumorigenic activity of cultures. Ann. N.Y. Acad. Sci. 76: 497-505, 1958. PubMed: 13627875 St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Gromeier M , et al. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93: 2370-2375, 1996. PubMed: 8637880 Roller RJ , et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758 Herold BC , et al. Differences in the susceptibility of herpes simplex virus types 1 and 2 to modified heparin compounds suggest serotype differences in viral entry. J. Virol. 70: 3461-3469, 1996. PubMed: 8648678 Chang YE , et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731 Carter KL , et al. Characterization of the products of the UL43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription. J. Virol. 70: 7663-7668, 1996. PubMed: 8892886 Carter KL , Roizman B . The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha22 gene. J. Virol. 70: 172-178, 1996. PubMed: 8523523 Jamaluddin M , et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70: 1554-1563, 1996. PubMed: 8627674 |
中文名称 | 人喉表皮样癌细胞 |
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