细胞名称 | C2C12小鼠成肌细胞 |
描述 | 这是D. Yaffe 和 O. Saxel建立的小鼠成肌细胞株的亚克隆(由H. Blau等人构建)。C2C12细胞株分化很快,形成可伸缩的肌管并生成特征性的肌蛋白。 用骨形成蛋白2(BMP-2)处理,导致分化途径从成肌细胞转换成成骨细胞。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 |
动物种别 | 小鼠 |
性别 | *** |
组织来源 | 组织: 肌肉 品系: C3H 细胞类型: 成肌细胞。 |
形态 | 成肌细胞 |
培养基和添加剂 | DMEM培养基(GIBCO,货号12800017,添加NaHCO3 1.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 |
供应限制 | A。仅供研究之用。 |
REFERENCE | Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524 Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846 Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324 Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
中文名称 | 小鼠成肌细胞 |
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