细胞名称

NIH/3T3小鼠胚胎细胞

描述

与原始的随机交配3T3和近交系BALB/c 3T3建株方法一样，NIH/3T3，是从NIH Swiss小鼠胚胎培养物中建立的高度接触抑制的连续细胞株。 为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了五轮以上亚克隆。 这株细胞对DNA转化及转染研究十分有用。 检测表明肢骨发育畸形病毒(鼠痘)阴性。

动物种别

小鼠

性别

***

组织来源

胚胎

形态

成纤维细胞

培养基和添加剂

DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

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