英文名称 | His tag(2D5) |
中文名称 | 小鼠抗聚组氨酸单克隆抗体 |
别 名 | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
说 明 书 | 0.1ml 0.2ml |
产品类型 | 标签抗体 |
研究领域 | 细胞生物 免疫学 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 2D5 |
交叉反应 | fusionprotein,HHHHHHtagged |
产品应用 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/1ml |
免 疫 原 | KLH conjugated HHHHHH peptide |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
生 产 商 | BIOSS |
PubMed | PubMed |
产品介绍 | background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。 2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物) 4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。 5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。 |
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