表1.不同温度下rTEV酶切活性
| 反应时间 | 不同温度下剪切活性(%) | |||
| 4°C | 16°C | 21°C | 30°C | |
| 1 h | 34 | 58 | 56 | 70 |
| 2 h | 58 | 80 | 78 | 90 |
| 3 h | 71 | 99 | 99 | 99 |
| 3.5h | 84 | 99 | 99 | 99 |
影响天然TEV酶活性的常见因素：1）PMSF和AEBSF（1 mM），TLCK（1 mM），Bestatin（1 mg/ml）、Pestatin A（1 mM），EDTA（1 mM）以及E-64（3 mg/ml），以上这些蛋白酶抑制剂对rTEV的活性没有影响；2）Zn离子（＞5 mM）对rTEV活性有抑制；3）与半胱氨酸反应的试剂也是rTEV的潜在抑制剂。
表达方式: A DNA sequence encoding the TEV Protease （NP_062908） （Gly 2038- Gly 2280） was fused with polyhistidine tag at the N-terminus, with a Ser 2256 Asn mutation
种属:Human
表达宿主: E.Coli
QC Testing
纯度:> 95%, as determined by SDS-PAGE
内毒素:< ﹤1.0 EU per 1μg cytokine as determined by the LAL method.
稳定性:Samples are stable for up to twelve months from date of receipt -70°C
预测N端: Met 1
分子量:The recombinant human TEV protease consists of 251 amino acids and has a predicted molecular mass of 28.6 kDa. As estimated in SDS-PAGE under reducing conditions.
缓冲液: Supplied as a 0.2μm filtered solution of 50mM Tris, 10%Sucrose,5mM DTT pH8.0
SDS-PAGE:
Usage Guide
储存方法:Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles.