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货号： bs-5696R-Bio 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-5696R-Bio
- 英文名称
- Anti-phospho-p107(Ser975)/Biotin
- 中文名称
- 生物素标记的磷酸化视网膜母细胞瘤样蛋白p107抗体
- 别 名
- RBL1(phospho S975); Cellular protein 107; CP107; PRB1; RBL1; Retinoblastoma like protein 1; RBL1_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 免疫学 信号转导 干细胞 细胞周期蛋白 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 产品应用
- WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 121kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Ser975
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
The pocket protein family consists of three structurally and functionally related proteins, Rb (retinoblastoma), p107, and p130. This family of tumor suppressors function to regulate important cellular transcription factors, such as the E2F family. The E2F proteins regulate the expression of genes whose products are important for cell cycle progression. The inactivation Rb is catalyzed by CDK phosphorylation thereby releasing E2F during the G1-S phase cellular progression. Unchecked inactivation of Rb in G1 phase has been indicated as a universal mechanism underlying cellular transformation . While Rb has been the most studied among the pocket proteins, p107 and p130 have also been shown to be key regulators of E2F. Several studies have also provided evidence that p107/p130 provide different functions in E2F regulation than does Rb. Rb, p107, and p130 each contain a conserved A/B pocket, which is the target of several viral oncoproteins, namely SV40 large T-antigen and adenovirus E1A. There are two isoforms.
Function:
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 Lys-20 trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Subunit:
Interacts with SUV420H1, SUV420H2 and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with SV40 and JC virus large T antigens.
Subcellular Location:
Nucleus.
Post-translational modifications:
Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
Similarity:
Belongs to the retinoblastoma protein (RB) family.
Database links:Entrez Gene: 5933 Human
Entrez Gene: 19650 Mouse
Entrez Gene: 680111 Rat
Omim: 116957 Human
SwissProt: P28749 Human
SwissProt: Q64701 Mouse
Unigene: 207745 Human
Unigene: 244671 Mouse
Unigene: 148725 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.