This DMEM/F-12 is modified as follows:
| With | Without |
| • L-glutamine | • Sodium Bicarbonate |
| • HEPES | |
| • Phenol Red |
The complete formulation is available.
Gibco® DMEM/F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids and vitamins with F-12s wide variety of components.
Product Intended Use
Forin vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM/F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system (2.438 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
内容及储存
储存条件：2-8° C
运输条件：环境温度
保存期：自生产日期起36个月