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货号： bs-14319R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-14319R
- 英文名称
- DHX36
- 中文名称
- DHX36蛋白抗体
- 别 名
- DDX36; DEAH box protein 36; Dhx36; DHX36_HUMAN; EC 3.6.1.-; KIAA1488; MLE like protein 1; MLE-like protein 1; MLEL1; Probable ATP dependent RNA helicase DHX36; Probable ATP-dependent RNA helicase DHX36; RHAU; RNA helicase associated with AU rich element ARE; RNA helicase associated with AU-rich element ARE.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 免疫学 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 115kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human DHX36:151-250/1008
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function:
Plays a role in degradation and deadenylation of mRNAs containing in their 3-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis.
Subcellular Location:
Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli.
Tissue Specificity:
Highly expressed in testis.
Similarity:
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
SWISS:
Q9H2U1
Gene ID:
170506
Database links:Entrez Gene: 170506Human
Entrez Gene: 72162Mouse
Omim: 612767Human
SwissProt: Q9H2U1Human
SwissProt: Q8VHK9Mouse
Unigene: 446270Human
Unigene: 224233Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-DHX36 (bs-14319R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-DHX36 (bs-14319R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (bs-14319R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.