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货号: bs-20188R 基本售价: 1580.0 元 规格: 100ul
产品信息
- 产品编号
- bs-20188R
- 英文名称
- ALPPL2
- 中文名称
- 胎盘碱性磷酸酶抗体
- 别 名
- Akp5;Alkaline phosphatase;Alkaline phosphatase Nagao isozyme;Alkaline phosphatase placental like;ALP 1;ALP-1;ALP1;ALPG;ALPPL 2;ALPPL;C77216;D1Ertd816e;GCAP;Germ cell alkaline phosphatase;Nagao isozyme;Placental alkaline phosphatase-like;Placental like alkaline phosphatase;placental-like;PLAP like;PLAP-like;PPBN_HUMAN;Testicular and thymus alkaline phosphatase. 小肠碱性磷酸酶;
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 激酶和磷酸酶 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 53kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ALPPL2:301-400/532
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]
Subunit:
Homodimer.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity:
Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
Similarity:
Belongs to the alkaline phosphatase family.
SWISS:
P10696
Gene ID:
251
Database links:Entrez Gene: 251 Human
Omim: 171810 Human
SwissProt: P10696 Human
Unigene: 333509 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
- Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ALPPL2 Polyclonal Antibody, Unconjugated(bs-20188R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingBlank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line):Rabbit Anti-ALPPL2 antibody (bs-20188R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.