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货号： bs-19402R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-19402R
- 英文名称
- PRP18 homolog
- 中文名称
- PRP18蛋白抗体
- 别 名
- FLJ10210; hPRP18; Pre mRNA splicing factor 18; Pre-mRNA-splicing factor 18; PRP18; PRP18 homolog; PRP18 pre mRNA processing factor 18 homolog (PRPF18); PRP18 pre mRNA processing factor 18 homolog (S. cerevisiae); PRP18_HUMAN; prpf18.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 40kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human PRP18 homolog:281-342/342
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]
Function:
Participates in the second step of pre-mRNA splicing.
Subunit:
Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs.
Subcellular Location:
Nucleus speckle. Colocalizes with spliceosomal snRNPs.
Similarity:
Belongs to the PRP18 family.
SWISS:
Q99633
Gene ID:
8559
Database links:Entrez Gene: 507639Cow
Entrez Gene: 477998Dog
Entrez Gene: 100727386 Guinea pig
Entrez Gene: 100056558Horse
Entrez Gene: 8559Human
Entrez Gene: 67229Mouse
Entrez Gene: 100513617Pig
Entrez Gene: 100340516Rabbit
Entrez Gene: 171552Rat
Omim: 604993Human
SwissProt: Q2HJ41Cow
SwissProt: Q99633Human
SwissProt: Q8BM39Mouse
SwissProt: Q9JKB8Rat
Unigene: 161181Human
Unigene: 38529Mouse
Unigene: 203914Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-PRP18 homolog (bs-19402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRP18 homolog) Polyclonal Antibody, Unconjugated (bs-19402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.