产品中心
当前位置：首页>产品中心Anti-CIDEC
货号： bs-6796R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-6796R
- 英文名称
- CIDEC
- 中文名称
- 细胞死亡活化蛋白抗体
- 别 名
- Cell Death Activator; Cell death activator CIDE-3; Cell Death Inducing DFFA Like Effector C; Cell death inducing DFFA like effector protein C; Cell death-inducing DFFA-like effector protein C; CIDE 3; CIDE3; CIDE C; CIDEC_HUMAN; Fat specific protein 27; Fat-specific protein FSP27 homolog; FLJ20871; FSP27.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 细胞生物 免疫学 信号转导 细胞凋亡 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 27kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CIDEC:101-200/238
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010].
Tissue specificity: Expressed mainly in small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver.
Function:
May act as a CEBPB coactivator in white adipose tissueto control the expression of a subset of CEBPB downstream targetgenes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (Bysimilarity). Binds to lipid droplets and regulates theirenlargement, thereby restricting lipolysis and favoring storage. Atfocal contact sites between lipid droplets, promotes directionalnet neutral lipid transfer from the smaller to larger lipiddroplets. The transfer direction may be driven by the internalpressure difference between the contacting lipid droplet pair. Whenoverexpressed in preadipocytes, induces apoptosis or increases cellsusceptibility to apoptosis induced by serum deprivation or TGFBtreatment. As mature adipocytes, that express high CIDEC levels,are quite resistant to apoptotic stimuli, the physiologicalsignificance of its role in apoptosis is unclear.
Subunit:
Interacts with CEBPB (By similarity). Interacts withCIDEA.
Subcellular Location:
Nucleus (By similarity). Endoplasmicreticulum (By similarity). Lipid droplet. Note=Diffuses quickly onlipid droplet surface, but becomes trapped and clustered at lipiddroplet contact sites, thereby enabling its rapid enrichment atlipid droplet contact sites.
Tissue Specificity:
Expressed mainly in adipose tissue, smallintestine, heart, colon and stomach and, at lower levels, in brain,kidney and liver.
Post-translational modifications:
Ubiquitinated and targeted to proteasomal degradation,resulting in a short half-life. Protein stability depends ontriaclyglycerol synthesis, fatty acid availability and lipiddroplet formation (By similarity).
DISEASE:
Note=In omental adipose tissue of obese patients matchedfor BMI, expression levels tend to correlate with insulinsensitivity. Expression is increased 2-3 fold in the group ofpatients with high insulin sensitivity, compared to theinsulin-resistant group. This observation is consistent with theidea that triglyceride storage in adipocytes plays an importantrole in sequestering triglycerides and fatty acids away from thecirculation and peripheral tissues, thus enhancing insulinsensitivity in liver and muscle. This effect is not significant insubcutaneous adipose tissue (PubMed:18509062). In subcutaneousadipose tissue of diabetic patients, tends to negatively correlatewith body mass index and total fat mass, independently of insulinsensitivity (PubMed:18334488).
Similarity:
Contains 1 CIDE-N domain.
SWISS:
Q96AQ7
Gene ID:
63924
Database links:Entrez Gene: 63924 Human
Entrez Gene: 14311 Mouse
Entrez Gene: 500292 Rat
Omim: 612120 Human
SwissProt: Q96AQ7 Human
SwissProt: P56198 Mouse
SwissProt: Q5XI33 Rat
Unigene: 567562 Human
Unigene: 635072 Human
Unigene: 10026 Mouse
Unigene: 33794 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Epididymis (Mouse) Lysate at 40 ug
Primary: Anti-CIDEC (bs-6796R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining