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货号： bs-7552R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-7552R
- 英文名称
- Biglycan
- 中文名称
- 骨/软骨蛋白多糖1抗体
- 别 名
- BGN; Biglycan; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A.
- Specific References (2) | bs-7552R has been referenced in 2 publications.[IF=1.72] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014). IHC-P ; Rabbit.PubMed:24987434[IF=5.23] Han, Chao, et al. "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." Scientific Reports 6 (2016): 35102. WB ; Rabbit.PubMed:27713567
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 信号转导 干细胞 细胞骨架 细胞外基质
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 37kDa
- 细胞定位
- 细胞外基质 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Biglycan:101-200/368
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
May be involved in collagen fiber assembly.
Tissue specificity; Found in several connective tissues, especially in articular cartilages.
Function:
May be involved in collagen fiber assembly (By similarity).
Subunit:
Homodimer. Forms a ternary complex with MFAP2 and ELN
Subcellular Location:
Secreted, extracellular space, extracellular.
Tissue Specificity:
Found in several connective tissues, especially in articular cartilages.
Post-translational modifications:
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity).
Similarity:
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
SWISS:
P21810
Gene ID:
633
Database links:Entrez Gene: 633 Human
Entrez Gene: 12111 Mouse
Entrez Gene: 25181 Rat
Omim: 301870 Human
SwissProt: P21810 Human
SwissProt: P28653 Mouse
SwissProt: P47853 Rat
Unigene: 821 Human
Unigene: 2608 Mouse
Unigene: 783 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Bone (mouse) Lysate at 40 ug
Primary: Anti- Biglycan (bs-7552R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 52 kD
Sample:
Colon (Rat) Lysate at 40 ug
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 37 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (bs-7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human endothelial cells;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:50, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Rhodamine B conjugated (bs-0295G-RBITC) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei