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货号： bsm-18133M-AF555 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bsm-18133M-AF555
- 英文名称
- Anti-MYLK2/AF555
- 中文名称
- AF555标记的肌球蛋白轻链激酶2单克隆抗体
- 别 名
- KMLC; MLCK; MLCK2; MYLK 2; Myosin light chain kinase 2; Myosin light chain kinase 2 skeletal muscle; Myosin light chain kinase 2 skeletal/cardiac muscle; Skeletal muscle myosin light chain kinase; Skeletal myosin light chain kinase; skMLCK; MYLK2_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 心血管 信号转导 激酶和磷酸酶 细胞骨架
- 抗体来源
- Mouse
- 克隆类型
- Monoclonal
- 克 隆 号
- 4F12
- 交叉反应
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 65kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 2mg/1ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human MYLK2
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.
Function:
Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
Subunit:
May interact with centrin.
Subcellular Location:
Cytoplasmic. Co-localizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.
Tissue Specificity:
heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.
DISEASE:
Defects in MYLK2 are a cause of familial hypertrophic cardiomyopathy (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Database links:Entrez Gene: 85366 Human
Omim: 606566 Human
SwissProt: Q9H1R3 Human
Unigene: 86092 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.