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货号： bs-20502R-AF555 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-20502R-AF555
- 英文名称
- Anti-PFKL/AF555
- 中文名称
- AF555标记的6磷酸果糖激酶（肝型）抗体
- 别 名
- Phosphofructokinase, Liver; Phosphofructo-1-Kinase Isozyme B; 6-Phosphofructokinase Type B; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-B; PFK-L; ATP-Dependent 6-Phosphofructokinase, Liver Type; 6-Phosphofructokinase, Liver Type; Liver-Type 1-Phosphofructokinase; EC 2.7.1; PFKAL_HUMAN;
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 信号转导 激酶和磷酸酶 合成与降解 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 85kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 2mg/1ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human PFKL
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
6-phosphofructokinase, liver type (PFKL) is an enzyme that in humans is encoded by the PFKL gene on chromosome 21. This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Function:
This gene encodes one of three protein subunits of PFK, which are expressed and combined to form the tetrameric PFK in a tissue-specific manner. As a PFK subunit, PFKL is involved in catalyzing the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate. This irreversible reaction serves as the major rate-limiting step of glycolysis. Notably, knockdown of PFKL has been shown to impair glycolysis and promote metabolism via the pentose phosphate pathway. Moreover, PFKL regulates NADPH oxidase activity through the pentose phosphate pathway and according to NADPH levels.
Subunit:
Homo- and heterotetramers. Muscle is M4, liver is L4, and red cell is M3L, M2L2, or ML3.
Subcellular Location:
Cytoplasm
Post-translational modifications:
GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation.
Similarity:
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.
Database links:Entrez Gene: 5211 Human
Entrez Gene: 18641 Mouse
Entrez Gene: 25741 Rat
Omim: 171860 Human
SwissProt: P17858 Human
SwissProt: P12382 Mouse
SwissProt: P30835 Rat
Unigene: 255093 Human
Unigene: 269649 Mouse
Unigene: 4212 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸果糖激酶又叫6-磷酸果糖激酶-1（英语Phosphofructokinase；PFK）是一类激酶，可作用于果糖-6-磷酸 (fructose 6-phosphate)。在糖酵解中和己糖激酶、丙酮酸激酶一样催化的都是不可逆反应，因此这三种酶都有调节糖酵解途径的作用。2,6-二磷酸果糖是它的最强变构激活剂。