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货号： bs-18275R-AF555 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-18275R-AF555
- 英文名称
- Anti-LIME/AF555
- 中文名称
- AF555标记的LCK相互作用膜蛋白抗体
- 别 名
- dJ583P15.4; FLJ20406; LIME1_HUMAN; Lck interacting membrane protein; Lck interacting molecule; Lck interacting transmembrane adaptor 1; LIME 1; LIME1; LP8067; RP4 583P15.5.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 免疫学 t-淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig, Cow, Horse,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 32kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human LIME
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]).[supplied by OMIM, Mar 2008]
Function:
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
Subcellular Location:
Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell
Database links:Entrez Gene: 54923Human
Omim: 609809Human
SwissProt: Q9H400Human
Unigene: 233220Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.