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货号： bs-16798R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-16798R
- 英文名称
- KMT5A
- 中文名称
- 组蛋白H4 K20甲基转移酶抗体
- 别 名
- H4 K20 HMTase; H4 K20 specific histone methyltransferase; H4-K20-HMTase SETD8; Histone H4 K20 methyltransferase; Histone lysine N methyltransferase H4 lysine 20 specific; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; N-lysine methyltransferase SETD8; Pr SET 7; Pr SET7; PR-Set7; PR/SET domain containing protein 07; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PrSET7; SET 07; SET 8; SETD8_HUMAN; SET domain containing (lysine methyltransferase) 8; SET domain containing 8; SET domain containing lysine methyltransferase 8; SET domain containing protein 8; SET domain-containing protein 8; SET07; SET8; SETD 8.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 甲基化抗体
- 研究领域
- 细胞生物 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 43kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human KMT5A:201-300/393
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates Lys-20 of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at Lys-382, leading to repress p53/TP53-target genes.
Subcellular Location:
Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
Similarity:
Belongs to the histone-lysine methyltransferase family.
PR/SET subfamily.
Contains 1 SET domain.
SWISS:
Q9NQR1
Gene ID:
387893
Database links:Entrez Gene: 387893Human
Entrez Gene: 67956Mouse
Entrez Gene: 689820Rat
Omim: 607240Human
SwissProt: Q9NQR1Human
SwissProt: Q2YDW7Mouse
Unigene: 443735Human
Unigene: 572262Human
Unigene: 137966Mouse
Unigene: 8879Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-KMT5A (bs-16798R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Blank control (blue line): Hela(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-KMT5A antibody (bs-16798R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.