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货号： bs-16796R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-16796R
- 英文名称
- KMT3C
- 中文名称
- 组蛋白赖氨酸N-甲基转移酶3C抗体
- 别 名
- Histone methyltransferase SMYD2; HSKM B; HSKM-B; HSKMB; KMT3C; Lysine N-methyltransferase 3C; MGC119305; N lysine methyltransferase SMYD2; N-lysine methyltransferase SMYD2; SET and MYND domain containing 2; SET and MYND domain containing protein 2; SET and MYND domain-containing protein 2; Smyd2; SMYD2_HUMAN; Zinc finger MYND domain containing 14; ZMYND14.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 甲基化抗体
- 研究领域
- 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Horse, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 50kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human KMT3C:351-433/433
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]
Function:
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 Lys-4 (H3K4me) and dimethylates histone H3 Lys-36 (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates Lys-370 of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates Lys-860 of RB1/RB.
Subunit:
Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1 (By similarity). Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1.
Subcellular Location:
Cytoplasm; cytosol. Nucleus.
Similarity:
Contains 1 MYND-type zinc finger.
Contains 1 SET domain.
SWISS:
Q9NRG4
Gene ID:
56950
Database links:Entrez Gene: 56950Human
Entrez Gene: 615229Cow
Entrez Gene: 226830Mouse
Entrez Gene: 100294706Pig
Entrez Gene: 289372Rat
Omim: 610663Human
SwissProt: Q0P585Cow
SwissProt: Q9NRG4Human
SwissProt: Q8R5A0Mouse
SwissProt: C3RZA1Pig
SwissProt: Q7M6Z3Rat
Unigene: 66170Human
Unigene: 156895Mouse
Unigene: 7052Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: heart (mouse) Lysate at 40 ug
Primary: Anti-KMT3C(bs-16796R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Tissue/cell: Human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-KMT3C Polyclonal Antibody, Unconjugated(bs-16796R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining