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货号： bs-5638R-AF555 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-5638R-AF555
- 英文名称
- Anti-Phospho-SHP2 (Tyr81)/AF555
- 中文名称
- AF555标记的磷酸化蛋白酪氨酸磷酸酶2抗体
- 别 名
- SYP(phospho Y81); SHIP2; BPTP 3; BPTP3; CFC; MGC14433; Noonan syndrome 1; Noonan syndrome 1 protein tyrosine phosphatase 2C; NS 1; NS1; Protein tyrosine phosphatase 2C; Protein Tyrosine Phosphatase Non receptor Type 11; PTP 1D; PTP 2C; PTP1D; PTP2C; PTPN 11; PTPN11; SAP2; SH PTP2; SH PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHIP2; SHP 2; SHP-2; SHPTP 2; SHPTP2; SHPTP3; SIT protein precursor; Syp; Tyrosine protein phosphatase non receptor type 11; Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 68kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human SHP2 around the phosphorylation site of 81
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non transmembrane PTP, designated SHPTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N terminal to the PTP domain. A second and much more widely expressed PTP with SH2 domains, SHPTP2 (also designated PTP1D and Syp), has been identified. SHP2 is a protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell signaling events that are important for many cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
Function:
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.
Subunit:
Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with FLT1 (tyrosine-phosphorylated), FLT3 (tyrosine-phosphorylated), FLT4 (tyrosine-phosphorylated), KIT and GRB2. Interacts with PDGFRA (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL3, FCRL4, FCRL6 and ANKHD1. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts with GAB2. Interacts with TERT; the interaction retains TERT in the nucleus. Interacts with PECAM1 and FER. Interacts with EPHA2 (activated); participates in PTK2/FAK1 dephosphorylation in EPHA2 downstream signaling. Interacts with ROS1; mediates PTPN11 phosphorylation. Interacts with PDGFRB (tyrosine phosphorylated); this interaction increases the PTPN11 phosphatase activity.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Post-translational modifications:
Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
DISEASE:
Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
Database links:Entrez Gene: 5781Human
Entrez Gene: 19247Mouse
Entrez Gene: 25622Rat
Omim: 176876Human
SwissProt: Q06124Human
SwissProt: P35235Mouse
SwissProt: P41499Rat
Unigene: 506852Human
Unigene: 474046Mouse
Unigene: 8681Mouse
Unigene: 98209Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
SHP2(SH-PTP2)参与多种细胞内信号传导 如MAP kinase、 PI3k等途径，SHP2也是许多其他原癌基因信号通路的重要组成部分，在细胞的增殖及分化等过程扮演重要的角色.