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货号： bs-7779R-AF555 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-7779R-AF555
- 英文名称
- Anti-BRD7/AF555
- 中文名称
- AF555标记的鼻咽癌相关转录调节蛋白抗体
- 别 名
- 75 kDa bromodomain protein; BP75; BRD 7; BRD7; BRD7_HUMAN; Bromodomain containing 7; bromodomain containing protein 7; Bromodomain-containing protein 7; CELTIX 1; CELTIX1; NAG4; Protein CELTIX-1.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 信号转导 细胞周期蛋白 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 74kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human BRD7
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.
Function:
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at Tyr-216. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at Lys-9 (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at Lys-382, and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Subunit:
Interacts with TRIM24, PTPN13 and DVL1. Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D and ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex (By similarity). Interacts with IRF2 and HNRPUL1. Interacts (via N-terminus) with TP53. Interacts (via C-terminus) with EP300. Interacts with BRCA1. Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at Lys-14 (H3K14ac). Has low affinity for histone H3 acetylated at Lys-9 (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at Lys-9 (H3K9ac) and at Lys-14 (H3K14ac). Has very low affinity for non-acetylated histone H3. Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at Lys-8 (H3K8ac) (in vitro).
Subcellular Location:
Isoform 2: Nucleus.
Similarity:
Contains 1 bromo domain.
Database links:
UniProtKB/Swiss-Prot: Q9NPI1.1
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
BRD7可能参与了基因转录调控,是一个核转录调节蛋白。经研究发现BRD7可能是通过Ras/MEK/ERK和Rb/E2F两条通路影响细胞周期的进程，使细胞停滞于G0/G1期，BRD7具有阻止细胞周期进程的作用。BRD7蛋白尤其是在鼻咽癌的发病过程中发挥了十分重要作用，BRD7基因的过表达可通过调节MAPK及Wnt信号通路抑制鼻咽癌的生长，并将鼻咽癌阻滞在G1/S期。