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货号： bs-9267R-AF555 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-9267R-AF555
- 英文名称
- Anti-USP10/AF555
- 中文名称
- AF555标记的去泛素酶10抗体
- 别 名
- Deubiquitinating enzyme 10; Ubiquitin carboxyl terminal hydrolase 10; Ubiquitin carboxyl-terminal hydrolase 10; Ubiquitin specific peptidase 10; Ubiquitin specific processing protease 10; Ubiquitin specific protease 10; Ubiquitin thioesterase 10; Ubiquitin thiolesterase 10; Ubiquitin-specific-processing protease 10; UBP10_HUMAN; UBPO; USP 10; USP10.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 87kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human USP10
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013].
Function:
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
Subunit:
Interacts with G3BP, which may regulate its function. Interacts with p53/TP53, SNX3 and CFTR.
Subcellular Location:
Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
Tissue Specificity:
Widely expressed.
Post-translational modifications:
Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Ubiquitinated. Deubiquitinated by USP13.
Similarity:
Belongs to the peptidase C19 family. USP10 subfamily.
Database links:Entrez Gene: 9100Human
Entrez Gene: 479625Dog
Entrez Gene: 22224Mouse
Entrez Gene: 307905Rat
Omim: 609818Human
SwissProt: Q14694Human
SwissProt: P52479Mouse
SwissProt: Q3KR59Rat
Unigene: 19403Dog
Unigene: 136778Human
Unigene: 256910Mouse
Unigene: 421337Mouse
Unigene: 24862Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.