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货号： bs-14512R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-14512R
- 英文名称
- EFCAB4B
- 中文名称
- EFCAB4B蛋白抗体
- 别 名
- EFCAB-4B;Calcium release-activated calcium channel regulator 2A; CRAC channel regulator 2A; CRACR2A; EF hand calcium binding domain 4B; EF hand calcium binding domain containing protein 4B; EF-hand calcium-binding domain-containing protein 4B; EFC4B_HUMAN; EFCAB 4B; Efcab4b; FLJ33805; MGC4266.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 信号转导 转运蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Horse, Rabbit, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 46/83kDakDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human EFCAB4B:201-300/1503
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
EF-CAB4B is a 395 amino acid protein belonging to the EF-CAB4 family. Localizing to cytoplasm, EF-CAB4B contains two EF-hand domains and exists as two alternatively spliced isoforms. At low Ca2+ concentrations, EF-CAB4B acts as a calcium-sensor, facilitating the clustering of Orai1 and Stim1 at the junctional regions between plasma membrane and endoplasmic reticulum, leading to regulation of CRAC channel activation. The gene encoding EF-CAB4B maps to human chromosome 12p13.32. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia.
Function:
Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the EFCAB4 family.
Contains 2 EF-hand domains.
SWISS:
Q9BSW2
Gene ID:
74766
Database links:Entrez Gene: 84766Human
Entrez Gene: 381812Mouse
Omim: 614178Human
SwissProt: Q9BSW2Human
SwissProt: Q3UP38Mouse
Unigene: 504534Human
Unigene: 296093Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-EFCAB4B (bs-14512R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/83 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EFCAB4B) Polyclonal Antibody, Unconjugated (bs-14512R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.