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货号： bs-14387R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-14387R
- 英文名称
- DNAJC21
- 中文名称
- DNAJC21蛋白抗体
- 别 名
- DJC21_HUMAN; DnaJ homolog subfamily A member 5; DnaJ homolog subfamily C member 21; DNAJA5; dnajc21; GS3; JJJ1; Protein GS3.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 神经生物学 信号转导 转运蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 62kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human DNAJC21:11-120/531
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
With the presence of the J domain defining a protein as a member, the DnaJ family has evolved with diverse cellular localization and functions and is one of the largest chaperone families. DnaJ heat-shock-induced proteins are derived from the bacterium Escherichia coli and are controlled by the htpR regulatory protein. DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. Members of this family contain cysteine-rich regions composed of zinc fingers that form a peptide-binding domain responsible for chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DNAJC21 (DnaJ homolog subfamily C member 21), also known as DNAJA5 or JJJ1, is a 531 amino acid protein that contains two C2H2-type zinc fingers and one J domain. Expressed in placenta, pancreas, kidney and brain, DNAJC21 may be a co-chaperone for HSP 70.
Function:
May act as a co-chaperone for HSP70.
Tissue Specificity:
Brain, placenta, kidney and pancreas.
Similarity:
Contains 2 C2H2-type zinc fingers.
Contains 1 J domain.
SWISS:
Q5F1R6
Gene ID:
134218
Database links:Entrez Gene: 134218Human
SwissProt: Q5F1R6Human
Unigene: 131887Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
293T (human)Cell Lysate at 40 ug
Cerebrum (mouse) Lysate at 40 ug
kidney (mouse) Lysate at 40 ug
pancreas (mouse) Lysate at 40 ug
Primary: Anti- DNAJC21(bs-14387R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNAJC21) Polyclonal Antibody, Unconjugated (bs-14387R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.