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货号： bs-15493R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-15493R
- 英文名称
- IDO
- 中文名称
- 吲哚胺2,3-双加氧酶抗体
- 别 名
- Indoleamine 2, 3-dioxygenase ; 3-dioxygenase; I23O1_HUMAN; IDO 1; IDO; IDO-1; IDO1; INDO; indolamine 2,3 dioxygenase; Indole 2 3 dioxygenase; indoleamine 2 3 dioxygenase 1; indoleamine 2 3 dioxygenase; Indoleamine 2,3-dioxygenase 1; Indoleamine pyrrole 2 3 dioxygenase; Indoleamine-pyrrole 2; indoleamine-pyrrole 2,3 dioxygenase.
- Specific References (2) | bs-15493R has been referenced in 2 publications.[IF=1.96] Fu, Jingjing, et al. "Effect of bone marrow-derived CD11b+ F4/80+ immature dendritic cells on the balance between pro-inflammatory and anti-inflammatory cytokines in DBA/1 mice with collagen-induced arthritis." Inflammation Research (2014): 1-11. Mouse.PubMed:24458308[IF=0.70] Jiang, N., et al. "Expression of indoleamine 2, 3-dioxygenase in a murine model of Aspergillus fumigatus keratitis."International Journal of Ophthalmology 9.4 (2016). IF(IHC-P) ; Mouse.PubMed:27162718
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 细胞生物 免疫学 信号转导 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 45kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human IDO:101-200/403
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes indoleamine 2,3-dioxygenase (IDO) – a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.
Function:
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
Similarity:
Belongs to the indoleamine 2,3-dioxygenase family.
SWISS:
P14902
Gene ID:
3620
Database links:Entrez Gene: 3620Human
Entrez Gene: 15930Mouse
Entrez Gene: 66029Rat
Omim: 147435Human
SwissProt: P14902Human
SwissProt: P28776Mouse
SwissProt: Q9ERD9Rat
Unigene: 840Human
Unigene: 392Mouse
Unigene: 64513Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IDO Polyclonal Antibody, Unconjugated(bs-15493R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, AF647 conjugated(bs-0295G-A647)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control(blue):Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti- IDO antibody(bs-15493R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.