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货号： bs-13282R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-13282R
- 英文名称
- GAPDHS
- 中文名称
- 精子3-磷酸甘油醛脱氢酶-2抗体
- 别 名
- GAPDH2; GAPD2; GAPDH 2; GAPDH 2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; G3PT_HUMAN; HSD-35.
- Specific References (1) | bs-13282R has been referenced in 1 publications.[IF=2.09] Zhang, Jizheng, Hua Zhang, and Tingting Zi. "Overexpression of microRNA-141 relieves chronic constriction injury-induced neuropathic pain via targeting high-mobility group box 1." International Journal of Molecular Medicine. WB ; Rat.PubMed:26398163
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 信号转导 细胞凋亡 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 45kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GAPDHS:251-350/408
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a protein belonging to theglyceraldehyde-3-phosphate dehydrogenase family of enzymes thatplay an important role in carbohydrate metabolism. Like its somaticcell counterpart, this sperm-specific enzyme functions in anicotinamide adenine dinucleotide-dependent manner to removehydrogen and add phosphate to glyceraldehyde 3-phosphate to form1,3-diphosphoglycerate. During spermiogenesis, this enzyme may playan important role in regulating the switch between differentenergy-producing pathways, and it is required for sperm motilityand male fertility. [provided by RefSeq, Jul 2008].
Function:
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
Subunit:
Homotetramer. Interacts with ARRB2; the interaction isdetected in the nucleus upon OR1D2 stimulation.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Testis specific.
Similarity:
Belongs to the glyceraldehyde-3-phosphatedehydrogenase family.
SWISS:
O14556
Gene ID:
26330
Database links:Entrez Gene: 26330Human
Entrez Gene: 14447Mouse
Entrez Gene: 66020Rat
Entrez Gene: 532231Cow
Omim: 609169Human
SwissProt: Q2KJE5Cow
SwissProt: O14556Human
SwissProt: Q64467Mouse
SwissProt: Q9ESV6Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-GAPDHS (bs-13282R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated(bs-13282R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining