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货号： bs-4209R-AF350 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-4209R-AF350
- 英文名称
- Anti-Cullin 2/AF350
- 中文名称
- AF350标记的Cullin2抗体
- 别 名
- Cullin2; Cullin-2; CUL 2; MGC131970; CUL2_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 细胞周期蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow, Horse,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 87kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Cullin 2
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
Function:
Core component of multiple cullin-RING-based ECS(ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligasecomplexes, which mediate the ubiquitination of target proteins. Mayserve as a rigid scaffold in the complex and may contribute tocatalysis through positioning of the substrate and theubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligaseactivity of the complex is dependent on the neddylation of thecullin subunit and is inhibited by the association of thedeneddylated cullin subunit with TIP120A/CAND1 (By similarity). Thefunctional specificity of the ECS complex depends on the substraterecognition component. ECS(VHL) mediates the ubiquitination ofhypoxia-inducible factor (HIF).
Subunit:
Component of multiple ECS (Elongin BC-CUL2/5-SOCS-boxprotein) E3 ubiquitin-protein ligase complexes formed of CUL2,Elongin BC (TCEB1 and TCEB2), RBX1 and a variablesubstrate-specific adapter. Component of the ECS(VHL) or CBC(VHL)complex containing VHL. Component of the ECS(MED8) complex with theprobable substrate recognition component MED8 (By similarity).Component of the ECS(LRR1) complex with the probable substraterecognition component LRR1. Component of a probable ECS E3ubiquitin-protein ligase complex containing CUL2, RBX1, TCEB1,TCEB2 and FEM1B. Part of an E3 ubiquitin-protein ligase complexincluding ZYG11B, CUL2 and Elongin BC. Part of an E3ubiquitin-protein ligase complex including ZYG11BL, CUL2 andElongin BC. Interacts with RBX1, RNF7, FEM1B and TIP120A/CAND1.Interacts with COPS2, and MED8 (By similarity). Interacts withhuman respiratory syncytial virus (HRSV) protein NS1. Found in acomplex composed of LIMD1, VHL, EGLN1/PHD2, TCEB2 AND CUL2.
Post-translational modifications:
CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Similarity:
Belongs to the cullin family.
Database links:Entrez Gene: 535219Cow
Entrez Gene: 477952Dog
Entrez Gene: 101152077Gorilla
Entrez Gene: 100054982Horse
Entrez Gene: 8453Human
Entrez Gene: 100395340 Marmoset (common)
Entrez Gene: 101051445Monkey
Entrez Gene: 71745Mouse
Entrez Gene: 100018154Opossum
Entrez Gene: 100174361Orangutan
Entrez Gene: 100512186Pig
Entrez Gene: 100074001 Platypus (Ornithorhynchus anatinus)
Entrez Gene: 100343513Rabbit
Entrez Gene: 361258Rat
Entrez Gene: 100550749Turkey
Entrez Gene: 336088Zebrafish
Omim: 603135Human
SwissProt: Q13617Human
SwissProt: Q9D4H8Mouse
SwissProt: Q5RCF3Orangutan
Unigene: 82919Human
Unigene: 291707Mouse
Unigene: 443148Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.