产品中心
当前位置：首页>产品中心Anti-TEM1
货号： bs-2101R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2101R
- 英文名称
- TEM1
- 中文名称
- 内涎蛋白/内皮唾液酸蛋白抗体
- 别 名
- Tumor endothelial marker 1 precursor; TEM 1; TEM-1; tumor endothelial marker 1; CD248; Endosialin; Tumor endothelial marker 1; CD164L1; MGC119478; MGC119479. CD164 sialomucin-like 1; CD248_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 细胞凋亡 生长因子和激素
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 80kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human TEM 1:251-350/757 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The transmembrane glycoprotein Tumor endothelial marker 1 (TEM1) is highly expressed in tumor endothelial cells however it is barely detectable on normal endothelial cells. It is believed to play a role in tumor angiogenesis. It is expressed in stromal fibroblasts, metastatic hepatic lesions and during angiogenesis of corpus luteum formation and wound healing. TEM1 is being investigated as a potential target for cancer treatment.
Function:
May play a role in tumor angiogenesis.
Subcellular Location:
Membrane; Single-pass type I membrane protein (Probable).
Tissue Specificity:
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
Post-translational modifications:
O-glycosylated with sialylated oligosaccharides.
May be N-glycosylated.
Similarity:
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.Contains 1 Sushi (CCP/SCR) domain.
SWISS:
Q9HCU0
Gene ID:
57124
Database links:Entrez Gene: 57124Human
Entrez Gene: 70445Mouse
Entrez Gene: 293669Rat
Omim: 606064Human
SwissProt: Q9HCU0Human
SwissProt: Q91V98Mouse
Unigene: 195727Human
Unigene: 29597Mouse
Unigene: 38806Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
内皮唾液酸蛋白（CD248）是近年发现的人类肿瘤标记物之一,在人类多种恶性肿瘤组织中异常高表达,内皮唾液酸蛋白对肿瘤细胞生长、血管生成、浸润及转移有重要作用。
- 产品图片
Sample:Thymus(mouse) Lysate at 40 ugPrimary: Anti-TEM1 (bs-2101R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 80 kDObserved band size: 130 kD
Sample: spleen(mouse) Lysate at 30 ug
Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 130 kD
Sample:
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 130 kD
Sample:
SW480(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti-TEM1 (bs-2101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 130 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEM1) Polyclonal Antibody, Unconjugated (bs-2101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.