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货号： bs-11458R-AF350 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-11458R-AF350
- 英文名称
- Anti-Ephexin-1/AF350
- 中文名称
- AF350标记的神经细胞鸟苷酸置换因子NGEF抗体
- 别 名
- ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 82kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human EPHEXIN (451-550aa)
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.
Function:
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Subunit:
Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase
Subcellular Location:
Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones.
Tissue Specificity:
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Post-translational modifications:
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
Database links:Entrez Gene: 25791Human
Entrez Gene: 53972Mouse
Entrez Gene: 246217Rat
Entrez Gene: 424744Chicken
Omim: 605991Human
SwissProt: Q8N5V2Human
SwissProt: Q8CHT1Mouse
SwissProt: Q5BKC9Rat
Unigene: 97316Human
Unigene: 435439Mouse
Unigene: 20670Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.