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货号： bs-23128R-APC 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-23128R-APC
- 英文名称
- Anti-CD163/APC
- 中文名称
- APC标记的CD163抗体
- 别 名
- Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 心血管 免疫学 细胞膜受体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 121kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 2mg/1ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD163
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.
Function:
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
Subcellular Location:
Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Tissue Specificity:
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Post-translational modifications:
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated.
Similarity:
Contains 9 SRCR domains.
Database links:Entrez Gene: 9332 Human
Entrez Gene: 93671 Mouse
Entrez Gene: 312701 Rat
Omim: 605545 Human
SwissProt: Q86VB7 Human
SwissProt: Q2VLH6 Mouse
Unigene: 504641 Human
Unigene: 37426 Mouse
Unigene: 203204 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD163是SRCR超家族成员之一，又称为血红蛋白清道夫受体（hemoglobin scavenger receptor, HbSR）、M130或p155。CD163的表达水平受很多因素调节，糖皮质激素及抗炎介质如IL-10以及IL-6能上调CD163, 而促炎介质如脂多糖（lipopolysaccharide, LPS）、γ-干扰素（interferon-γ， IFN-γ）以及TNF-α则抑制CD163表达。 CD163是一种I型膜蛋白，又称为M130抗原、Ber-Mac3、Ki-M8或SM4。CD163限制性表达于单核/巨噬细胞系，所有循环系统的单核细胞和大多数组织（除外淋巴滤泡套区和生发中心）的巨噬细胞均阳性表达，主要用于单核/巨噬细胞的检测。