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货号： bs-11200R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-11200R
- 英文名称
- LHX2
- 中文名称
- 调控胚胎干细胞分化蛋白Lhx2抗体
- 别 名
- hLhx2; Homeobox protein LH-2; Homeobox protein LH2; LH2; LHX2; LHX2_HUMAN; LIM homeobox 2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2.
- Specific References (1) | bs-11200R has been referenced in 1 publications.[IF=1.36] Geng, Rongqing, et al. "Cyclic expression of Lhx2 is involved in secondary hair follicle development in cashmere goat." Gene Expression Patterns (2014). IHC-P ; Goat.PubMed:25128627
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 神经生物学 干细胞 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 45kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human LHX2:251-330/406
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
During development, genetically distinct subtypes of motor neurons express unique combinations of LIM-type homeodomain factors, which regulate cell migration and guide motor axons to establish the fidelity of a binary choice in axonal trajectory. The LIM gene family encodes a set of gene products, which carry the LIM domain, a unique cysteine-rich zinc-binding domain. At least 40 members of this family have been identified in vertebrates and invertebrates, and are distributed into 4 groups according to the number of LIM domains and to the presence of homeodomains and kinase domains. The overlapping expression of LHX1, LHX3, LHX4, Isl-1 and Isl-2 in developing motor neurons along the spinal column may influence the establishment of specific motor neuron subtypes. The human LHX2 gene maps to chromosome 9q33.3 and encodes a 389 amino acid protein. LHX2 is involved in early patterning of the telencephalon, where the neuroepithelium is first divided into cortical tissue and cortical hem.
Function:
Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
Subunit:
Contains 1 homeobox DNA-binding domain.Contains 2 LIM zinc-binding domains.
Subcellular Location:
Nucleus.
Similarity:
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding omains.
SWISS:
P50458
Gene ID:
9355
Database links:Entrez Gene: 16870 Mouse
Entrez Gene: 296706 Rat
SwissProt: Q9Z0S2 Mouse
SwissProt: P36198 Rat
Unigene: 142856 Mouse
Unigene: 81063 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LHX2) Polyclonal Antibody, Unconjugated (bs-11200R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).