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货号： bs-11128R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-11128R
- 英文名称
- CASPR
- 中文名称
- 轴突蛋白4/少突胶质细胞抗体
- 别 名
- Neurexin4; caspr 1; Caspr; Caspr1; Cntnap 1; Cntnap1; CNTP 1; CNTP1; CNTP1_HUMAN; Contactin associated protein 1; Contactin-associated protein 1; MHDNIV; NCP 1; NCP1; Neurexin 4; Neurexin IV; Neurexin-4; Nrxn 4; Nrxn4; p190; Paranodin.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 神经生物学 信号转导 细胞粘附分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 154kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CASPR/Neurexin4:151-250/1384
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Neurexins comprise a family of neuronal cell surface proteins, which include neurexin I (NRXN1), neurexin II (NRXN2), neurexin III (NRXN3) and CASPR (neurexin IV). Neurexins I-III are expressed as å and ∫ isoforms. The å isoforms are made of three cassettes, which contain two LNS (laminin A, neurexins, sex hormone-binding)-domains separated by EGF domains, followed by a transmembrane region and a 55 amino acid cytoplasmic C-terminal. The å isoforms bind to neurexophilins at the second LNS site, and to the excitatory neurotoxin å-latrotoxin. The ∫ isoforms have only one LNS-domain, bind to neuroligins and play a role in the formation and remodeling of synapses. CASPR (for contactin-associated protein 1, also designated paranodin in mouse), contains an extracellular domain similar to the other three neurexins, and binds to the surface glycoprotein contactin. CASPR and the closely related CASPR2, a mammalian homolog of Drosophila neurexin IV (Nrx-IV), demarcate distinct subdomains in myelinated axons. Specifically, CASPR exists at the paranodal junctions, while CASPR2 co-localizes with Shaker-like K+ channels in the juxtaparanodal region. CASPR may play a role in the communication of glial cells and neurons during development.
Function:
Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
Subunit:
Interacts with contactin in cis form.
Subcellular Location:
Membrane.
Tissue Specificity:
Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
Similarity:
Belongs to the neurexin family.
SWISS:
P78357
Gene ID:
8506
Database links:Entrez Gene: 8506Human
Entrez Gene: 53321Mouse
Entrez Gene: 84008Rat
Omim: 602346Human
SwissProt: P78357Human
SwissProt: O54991Mouse
SwissProt: P97846Rat
Unigene: 408730Human
Unigene: 474527Mouse
Unigene: 88654Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Tissue/cell: human glioma cells(U251 cells);4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CASPR Polyclonal Antibody, Unconjugated(bs-11128R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit CASPR antibody (bs-11128R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.