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货号： bs-4917R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-4917R
- 英文名称
- Osteocalcin
- 中文名称
- 骨钙蛋白/骨钙素抗体
- 别 名
- Osteocalcin Propeptide; Osteocalcin; Osteocalcin precursor; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN.
- Specific References (3) | bs-4917R has been referenced in 3 publications.[IF=8.31] Li, Long, et al. "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." Biomaterials (2014). IHC-P ; Rat.PubMed:25453952[IF=4.15] Ding, Shan, et al. "A nano-micro alternating multilayer scaffold loading with rBMSCs and BMP-2 for bone tissue engineering." Colloids and Surfaces B: Biointerfaces (2015). IHC-P ; Rat.PubMed:26119373[IF=1.26] Bugueño, Juan, et al. "Canine mesenchymal stem cell bone regenerative capacity is regulated by site-specific multi-lineage differentiation." Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology (2016). WB ; Dog.PubMed:0
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 信号转导 干细胞 细胞外基质
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 11kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Osteocalcin:21-100/100
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.
Function:
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular Location:
Secreted.
Post-translational modifications:
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Similarity:
Belongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
SWISS:
P02818
Gene ID:
632
Database links:Entrez Gene: 281646 Cow
Entrez Gene: 632 Human
Entrez Gene: 397530 Pig
Entrez Gene: 25295 Rat
Omim: 112260 Human
SwissProt: P02820 Cow
SwissProt: P02818 Human
SwissProt: Q8HYY9 Pig
SwissProt: P04640 Rat
Unigene: 654541 Human
Unigene: 9722 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
骨钙素又称骨γ-羧谷氨酸包含蛋白
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (bs-0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.