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货号： bs-1714R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1714R
- 英文名称
- Dopamine Transporter
- 中文名称
- 多巴胺转运蛋白DAT抗体
- 别 名
- DA transporter; DAT 1; DAT; DAT1; Dopamine transporter; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR); dopamine transporter; ADAT 1; Adenosine deaminase tRNA specific 1; HADAT1; tRNA specific adenosine deaminase 1.
- Specific References (3) | bs-1714R has been referenced in 3 publications.[IF=3.31] Ni, Na, et al. "Self-Assembling Peptide Nanofiber Scaffolds Enhance Dopaminergic Differentiation of Mouse Pluripotent Stem Cells in 3-Dimensional Culture." PLOS ONE 8.12 (2013): e84504. IF(ICC) ; Mouse.PubMed:24376815[IF=0.64] Qian, Weibin, et al. "Effect of Gingerol on Cisplatin-Induced Pica Analogous to Emesis Via Modulating Expressions of Dopamine 2 Receptor, Dopamine Transporter and Tyrosine Hydroxylase in the Vomiting Model of Rats."Yonago Acta medica 59 (2016): 100-110. IHC-F ; Rat.PubMed:27493480[IF=2.57] Romei, Cristina, et al. "Colocalization of neurotransmitter transporters on the plasma membrane of the same nerve terminal may reflect cotransmission."Brain Research Bulletin (2016). IHC ; Mouse.PubMed:27565422
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 神经生物学 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 68kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human DAT1:251-350/620 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3 UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]
Function:
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Subunit:
Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2.
Subcellular Location:
Membrane; Multi-pass membrane protein.
DISEASE:
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
Similarity:
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
SWISS:
Q01959
Gene ID:
6531
Database links:Entrez Gene: 6531 Human
Entrez Gene: 13162 Mouse
Entrez Gene: 24898 Rat
Omim: 126455 Human
SwissProt: Q01959 Human
SwissProt: Q61327 Mouse
SwissProt: P23977 Rat
Unigene: 406 Human
Unigene: 41993 Mouse
Unigene: 10093 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
DATI 可能参与神经系统的多种活动，如学习记忆、嗅觉、感觉、运动、多巴胺神经递质活动的调节，而且可能参与胶质瘤等神经系统肿瘤的发生,阳性着色主要定位于细胞质，但也可见到有些细胞核内的阳性染色.
- 产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Dopamine Transporter (bs-1714R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (bs-1714R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (bs-1714R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: U937 (blue).
Primary Antibody:Rabbit Anti-Dopamine Transporter antibody(bs-1714R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-1714R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Positive control: RSC96
Isotype Control Antibody: rabbit IgG-PE/CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.
Positive control: RSC96
Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number: bs-1714R; Dilution: 12μg in 100 μl 1X PBS containing 0.5% BSA