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货号： bs-9702R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-9702R
- 英文名称
- HECA
- 中文名称
- HECA蛋白抗体
- 别 名
- HDCL; Gm869; HDC; HDC_HUMAN; Headcase homolog (Drosophila); Headcase protein homolog; HECA; hHDC.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 心血管 细胞生物 免疫学 神经生物学 细胞周期蛋白 细胞分化 肿瘤细胞生物标志物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 59kDa
- 细胞定位
- 细胞核 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human HECA:231-330/543
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
HECA, also known as headcase homolog, HDC, HDCL or HHDC, is a 543 amino acid mammalian homolog of the Drosophila headcase protein, a highly basic, cytplasmic peptide that plays a role in mitotic re-entry during adult morphogenesis. Expressed in a variety of tissues with highest expression in thymus, spleen and heart, HECA is thought to play a role in the development of epithelial tube networks in lung tissue and may also be involved in the pathogenesis of lung cancer. The gene encoding HECA maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinsons disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6.
Function:
May play an important role in some human cancers. May bepart of the regulatory mechanism in the development of epithelialtube networks such as the circulatory system and lungs.
Tissue Specificity:
Expressed in all tissues examined. Highest levels are in the spleen, thymus, peripheral blood and heart. Lowest in the kidney and pancreas.
SWISS:
Q9UBI9
Gene ID:
51696
Database links:Entrez Gene: 51696 Human
Entrez Gene: 380629 Mouse
Entrez Gene: 308624 Rat
Omim: 607977 Human
SwissProt: Q9UBI9 Human
Unigene: 197644 Human
Unigene: 473073 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Lung (Rat) Lysate at 40 ug
Primary: Anti-HECA (bs-9702R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HECA Polyclonal Antibody, Unconjugated(bs-9702R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining