Anti-Bax【科瑞奥博】

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Anti-Bax

货号： bs-0127R 基本售价： 380.0 元规格： 20ul

规格：20ul: 价格：380.00元

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-0127R

英文名称: Bax

中文名称: Bax抗体

别名: apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4.

: Specific References  (27)     |     bs-0127R has been referenced in 27 publications.
[IF=3.73] Rani, Neha, et al. "Regulation of Heat Shock Proteins 27 and 70, p-Akt/p-eNOS and MAPKs by Naringin Dampens Myocardial Injury and Dysfunction In Vivo after Ischemia/Reperfusion." PLOS ONE 8.12 (2013): e82577.  WB ;  Rat.
PubMed:24324809
[IF=3.33] Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.  WB ;  Human.
PubMed:23050783
[IF=3.33] Tian, Xiao Qiang, et al. "The protective effect of Hyperbaric oxygen and Ginkgo Biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats." Behavioural Brain Research (2012).  WB ;  Rat.
PubMed:23266522
[IF=3.06] Li, Guiying, et al. "Gremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway." Journal of Cellular Biochemistry (2013).  WB ;  Mouse.
PubMed:23553804
[IF=1.31] Wang, Chunqiang, Wei Ma, and Yuhong Su. "NF-κB Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells." Biological trace element research (2013): 1-8.  WB ;  Pig.
PubMed:23575899
[IF=2.27] He, X., et al. "Rapamycin inhibits acrolein‐induced apoptosis by alleviating ROS‐driven mitochondrial dysfunction in male germ cells." Cell Proliferation (2014).  WB ;  Mouse.
PubMed:24483236
[IF=2.75] Ye, Bengui, et al. "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." Journal of Ethnopharmacology (2014).  WB ;  Human.
PubMed:24583069
[IF=2.69] Lin, Caiyu, et al. "Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo." Nutrition and Cancer (2014).  WB ;  Human.
PubMed:24660968
[IF=3.73] Zhu, Zhongling, et al. "Regulation of Cell Transformation by Rb-Controlled Redox Homeostasis." PLOS ONE 9.7 (2014): e102582.  WB ;  Mouse.
PubMed:25019272
[IF=3.70] Lin, Lai-xiang, et al. "Feasibility of β-Sheet Breaker Peptide-H102 Treatment for Alzheimers Disease Based on β-Amyloid Hypothesis." PLoS one 9.11 (2014): e112052.  IHC-P ;  Mouse.
PubMed:25372040
[IF=1.14] Wang, J., et al. "Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway." Brazilian Journal of Medical and Biological Research (2014): 000-000.  WB ;  Mouse.
PubMed:25517918
[IF=2.38] She, Yue, et al. "Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats." Cell Biochemistry and Biophysics (2015): 1-9.  IHC-P ;  Rat.
PubMed:25578489
[IF=2.17] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014).  WB ;  Rabbit.
PubMed:25763091
[IF=4.47] Park, Min-Jung, et al. "New role of irisin in hepatocytes: The protective effect of hepatic steatosis in vitro." Cellular Signalling (2015).  WB ;  Mouse.
PubMed:25917316
[IF=0.88] Li, Jinglong, et al. "Deficits of learning and memory in Hemojuvelin knockout mice." Journal of Veterinary Medical Science (2015).  WB ;  Mouse.
PubMed:26027705
[IF=1.63] Aksu, E. H., et al. "Mitigation of paracetamol‐induced reproductive damage by chrysin in male rats via reducing oxidative stress." Andrologia (2016).  IHC-P ;  Rat.
PubMed:26914515
[IF=5.62] Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917.  IP ;  Human.
PubMed:25444901
[IF=1.51] Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608.  IHC-P ;  Pig.
PubMed:22023076
[IF=2.86] Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96.  WB ;  Mouse.
PubMed:26771609
[IF=1.01] Yang, H., et al. "Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro." Brazilian Journal of Medical and Biological Research 43.11 (2010): 1027-1033.  IF(ICC) ;  Human.
PubMed:20945038
[IF=3.56] He, Xin, et al. "Canonical Wnt signaling pathway contributes to the proliferation and survival in porcine pancreatic stem cells (PSCs)." Cell and tissue research 362.2 (2015): 379-388.  WB ;  Pig.
PubMed:26085341
[IF=3.23] Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.  WB ;  Human.
PubMed:27423629
[IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedebergs Archives of Pharmacology (2017): 1-17.  IHC-P ;  Rat.
PubMed:28070612
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.
PubMed:27665780
[IF=2.62] Rahman, Md Mostafizur, et al. "Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism." Chemico-Biological Interactions (2017).  WB ;  Rat.
PubMed:28390674
[IF=0.44] Sutrisno, Sutrisno, et al. "The effect of genistein on TGF-β signal, dysregulation of apoptosis, cyclooxygenase-2 pathway, and NF-kB pathway in mice peritoneum of endometriosis model." Middle East Fertility Society Journal (2017).  IHC-P ;  Mouse.
PubMed:0
[IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).  WB ;  Human.
PubMed:0

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 细胞生物信号转导细胞凋亡线粒体

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 21kDa

细胞定位: 细胞浆细胞膜线粒体

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human Bax:84-175/192

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].

Function:
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Subunit:
Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2.

Subcellular Location:
Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Isoform Beta: Cytoplasm.
Isoform Gamma: Cytoplasm.
Isoform Delta: Cytoplasm (Potential).

Tissue Specificity:
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitts lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Similarity:
Belongs to the Bcl-2 family.

SWISS:
Q07812

Gene ID:
581

Database links:

Entrez Gene: 581Human

Entrez Gene: 12028Mouse

Entrez Gene: 24887Rat

Omim: 600040Human

SwissProt: Q07812Human

SwissProt: Q07813Mouse

SwissProt: Q63690Rat

Unigene: 624291Human

Unigene: 19904Mouse

Unigene: 10668Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

可识别分子量为21KDa的Bax蛋白，此抗体与Bax有较高特异性，且与Bc1-2及Bc1-X蛋白无交叉反应，Bax、Bc1-2和Bc1-X蛋白是凋亡调节蛋白家庭成员。与Bc1-2和Bc1-X相反，Bax蛋白的过量表达加速细胞凋亡。Bax在组织中广泛表达。
Bax与Bc1-2比值的高低可用于判断恶性肿瘤耐药及复发。此抗体用于肿瘤及细胞凋亡等方面的研究。最新的研究表明：Bax可能具有肿瘤抑制作用。

产品图片: Sample: Brain(Rat)lysate 30ug;
Liver(Mouse) lysates, 30ug;
Primary: Anti-Bax (bs-0127R) at 1:200;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 21kD
Observed band size : 21kD
Sample:
Cerebral cortex (Rat) Lysate at 40 ug
Primary: Anti-Bax (bs-0127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Sample:
Hela-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Bax (bs-0127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 23 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Bax (bs-0127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:800, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Overlay histogram showing HL 60 cells stained with bs-0127R (Green line).
The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-0127R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.