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货号： bs-8486R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-8486R
- 英文名称
- FNDC5
- 中文名称
- Ⅲ型纤维连接蛋白域蛋白2/5抗体
- 别 名
- FNDC5; Fibronectin type III domain containing 5; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat containing protein 2; Fibronectin type III repeat-containing protein 2; FNDC 5; Fndc5; FNDC5_HUMAN; FRCP2; Irisin.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 免疫学 G蛋白信号
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 12/20kDa
- 细胞定位
- 细胞浆 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human FNDC5 Irisin:81-143/212 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013].
Function:
Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA (By similarity).
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein (By similarity). Note=Imported in peroxisomes through the PEX5 receptor pathway (By similarity).
Irisin: Secreted. Note=Detected in the blood of individuals subjected to endurance exercise.
Post-translational modifications:
The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.
Similarity:
Contains 1 fibronectin type-III domain.
SWISS:
Q8NAU1
Gene ID:
252995
Database links:Entrez Gene: 252995 Human
Entrez Gene: 384061 Mouse
Entrez Gene: 260327 Rat
Omim: 611906 Human
SwissProt: Q8NAU1 Human
SwissProt: Q8K4Z2 Mouse
SwissProt: Q8K3V5 Rat
Unigene: 524234 Human
Unigene: 44075 Mouse
Unigene: 18757 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
HepG2(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
K562(Human) Lysate at 30 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-FNDC5 (bs-8486R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/20 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FNDC5) Polyclonal Antibody, Unconjugated (bs-8486R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.