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货号： bs-7827R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-7827R
- 英文名称
- RCL
- 中文名称
- c-Myc应答蛋白抗体
- 别 名
- c Myc responsive; c Myc responsive protein Rcl; C6orf108; Chromosome 6 open reading frame 108; Putative c Myc responsive; RCL_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 细胞周期蛋白 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 19kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human RCL/c Myc responsive:101-174/174
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene was identified on the basis of its stimulationby c-Myc protein. The latter is a transcription factor thatparticipates in the regulation of cell proliferation,differentiation, and apoptosis. The exact function of this gene isnot known but studies in rat suggest a role in cellularproliferation and c-Myc-mediated transformation. Two alternativetranscripts encoding different proteins have been described.[provided by RefSeq, Jul 2008].
Function:
Catalyzes the cleavage of the N-glycosidic bond ofdeoxyribonucleoside 5-monophosphates to yield deoxyribose5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside5-monophosphates containing purine bases are preferred to thosecontaining pyrimidine bases (By similarity).
Subunit:
Monomer and homodimer (By similarity).
Subcellular Location:
Nucleus (By similarity). Cytoplasm.
Tissue Specificity:
Expressed at low levels in brain, colon, lung,peripheral blood leukocytes, placenta, small intestine, and thymus.Expressed at high levels in heart, kidney, liver, skeletal muscleand spleen. Overexpressed in a significant proportion of breastcancers.
Similarity:
Belongs to the deoxyribonucleoside 5-monophosphateN-glycosidase family
SWISS:
O43598
Gene ID:
10591
Database links:Entrez Gene: 10591 Human
SwissProt: O43598 Human
Unigene: 109752 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Kidney (Rat) Lysate at 40 ug
Liver (Rat) Lysate at 40 ug
Primary: Anti-RCL (bs-7827R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
We are unsure as to the identity of these extra bandsMay be formed of homo-dimer
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RCL/c Myc responsive Polyclonal Antibody, Unconjugated(bs-7827R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining