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货号： bs-13641R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-13641R-PE-Cy7
- 英文名称
- Anti-GRB 14/PE-Cy7
- 中文名称
- PE-Cy7标记的生长因子受体结合蛋白14抗体
- 别 名
- GRB14 adapter protein; GRB14; GRB14_HUMAN; Growth factor receptor bound protein 14.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 信号转导 激酶和磷酸酶 细胞膜受体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 61kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GRB 14
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phospho-tyrosine-containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N-terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. An additional member of the GRB7 family, designated GRB14, contains a Pleckstrin homology domain in its central region and a carboxy-terminal SH2 domain. GRB14 mRNA is expressed at high levels in a broad range of tissues including liver, kidney, pancreas, testis, ovary, heart and skeletal muscle. Expression of the GRB14 protein in breast carcinomas is strongly correlated with estrogen receptor positivity.
Function:
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date.
Subunit:
Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor (INSR), through the SH2 domain (By similarity). Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation. Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated).
Subcellular Location:
Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Endosome membrane.
Tissue Specificity:
Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
Post-translational modifications:
Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2.
Similarity:
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain.
Database links:Entrez Gene: 2888Human
Entrez Gene: 50915Mouse
Entrez Gene: 58844Rat
Omim: 601524Human
SwissProt: Q14449Human
SwissProt: Q9JLM9Mouse
SwissProt: O88900Rat
Unigene: 411881Human
Unigene: 214554Mouse
Unigene: 30028Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.