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货号： bs-0961R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0961R
- 英文名称
- Leptin receptor
- 中文名称
- 瘦素受体抗体
- 别 名
- obl; CD 295; CD295; CD295 antigen; Db; Fa; HuB219; LEP R; LEPR; LEPROT; Leptin receptor fatty; Leptin receptor gene related protein; Leptin receptor precursor; OB R; OB-R; OB R gene related protein; OB receptor; OB-RGRP; Obr; Ob-R a/b/c/d/e; LEPR_HUMAN; LEPRD; Leptin receptor.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 神经生物学 细胞膜受体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1µg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 130kDa
- 细胞定位
- 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Leptin receptor:501-600/1165 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
Function:
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
Subunit:
On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3. Interaction with SOCS3 inhibits LRb signaling.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Isoform E: Secreted (Probable).
Tissue Specificity:
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
Post-translational modifications:
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain.
SWISS:
P48357
Gene ID:
3953
Database links:Entrez Gene: 3953Human
Entrez Gene: 16847Mouse
Entrez Gene: 24536Rat
Omim: 601007Human
SwissProt: P48357Human
SwissProt: P48356Mouse
SwissProt: Q62959Rat
Unigene: 23581Human
Unigene: 723178Human
Unigene: 259282Mouse
Unigene: 9891Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Leptin是一种分子量为16kDa的脂肪组织源激素，由脂肪细胞分泌的，具有强亲水性，以单体形式存在于血浆中。Leptin 具有广泛的生物学效应，作用于下丘脑，调节食欲、能量代谢及体重。Leptin 还可能作为脂肪-胰岛内分泌轴一部分，参与胰岛素分泌的调节。Leptin receptor (OB-R)是与gp130最密切相关的信号转导的共同成分,属1类细胞因子超家族，下丘脑有丰富的Leptin receptor，在其它部位也有分布，但水平较低，如：胰岛素B细胞、脉络丛、肝、肺、心、肾、睾丸、淋巴细胞和脂肪细胞中。人类存在四个异性体，均为单跨膜受体。其中OB-Rb在胞内结构域最长，是唯一能进行信号传导并调节热量摄入和能量消耗的异性体,分子量为132kDa。
- 产品图片
Sample:
Liver(Mouse) Lysate at 40 ug
Primary: Anti-Leptin receptor (bs-0961R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 155 kD
Sample:
Liver (Rat) Lysate at 40 ug
Primary: Anti-Leptin receptor (bs-0961R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 160 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LEPR) Polyclonal Antibody, Unconjugated (bs-0961R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Leptin receptor Polyclonal Antibody, Unconjugated(bs-0961R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Primary Antibody:Rabbit Anti-Leptin receptor antibody(bs-0961R)
Isotype Control Antibody: Rabbit IgG(orange)Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE(white blue)
Positive control: K562 cells(Blue)( An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.)