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货号： bs-8929R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-8929R-PE-Cy7
- 英文名称
- Anti-Cyclin E1/PE-Cy7
- 中文名称
- PE-Cy7标记的周期素E1抗体
- 别 名
- CCNE 1; CCNE; CCNE1; Cyclin E1 antibody Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; CCNE1_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 神经生物学 细胞凋亡 细胞周期蛋白 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human,
- 产品应用
- Flow-Cyt=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 47kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Cyclin E
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Function:
Essential for the control of the cell cycle at the G1/S (start) transition.
Subunit:
Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.
Subcellular Location:
Nucleus.
Tissue Specificity:
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Post-translational modifications:
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the cyclin family. Cyclin E subfamily.
Database links:Entrez Gene: 898 Human
Entrez Gene: 12447 Mouse
Entrez Gene: 25729 Rat
Omim: 123837 Human
SwissProt: P24864 Human
SwissProt: Q61457 Mouse
SwissProt: P39949 Rat
Unigene: 244723 Human
Unigene: 16110 Mouse
Unigene: 15455 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
细胞周期素E是调控细胞G-1→S期转变的关键因素。由于在多种肿瘤中的不适当表达,细胞周期素E现在已明确为原癌基因。