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货号： bs-13119R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-13119R-PE-Cy7
- 英文名称
- Anti-Exonuclease 1/PE-Cy7
- 中文名称
- PE-Cy7标记的核酸外切酶1抗体
- 别 名
- exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangu
- 产品应用
- Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 94kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Exonuclease 1
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the Exo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/EXO1 gene to chromosome 1q43. Exo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5-3 double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The MMR system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MMR. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.
Function:
5->3 double-stranded DNA exonuclease which may also possess a cryptic 3->5 double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5 or 3 to the mismatch. Also exhibits endonuclease activity against 5-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Subunit:
Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5->3 exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3->5 exonuclease activity and suppress the 5->3 exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5->3 exonuclease activity and cleavage of 5-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5-overhanging flap structures.
Subcellular Location:
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
Tissue Specificity:
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Post-translational modifications:
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
Similarity:
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
Database links:Entrez Gene: 457856 Chimpanzee
Entrez Gene: 9156 Human
Entrez Gene: 305000 Rat
Omim: 606063 Human
SwissProt: Q9UQ84 Human
Unigene: 498248 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.