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货号： bs-13053R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-13053R-PE-Cy7
- 英文名称
- Anti-EED/PE-Cy7
- 中文名称
- PE-Cy7标记的胚胎外胚层发育蛋白EED抗体
- 别 名
- eed; EED protein; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, Xenopus laevis, Xenopus tropicalis , Medaka fish
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 50kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human EED
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. EED (embryonic ectoderm development) is the human homolog of Eed, a murine PcG gene homologous to the Drosophila homeotic gene, extra sex combs. The human EED protein is 99. 5% identical to the mouse EED protein and contains seven WD repeats, which are involved in protein-protein interactions. There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, 535 and 494 amino acids in length. EED interacts in a highly specific manner, both in vitro and in vivo, with histone deacetylase (HDAC) proteins.
Function:
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates Lys-9 and Lys-27 of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Subunit:
Interacts with MLL (By similarity). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. May interact with the MA protein of HIV-1.
Subcellular Location:
Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
Tissue Specificity:
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Post-translational modifications:
Methylated. Binding to histone H1 Lys-26 promotes mono-, di-, and trimethylation of internal lysines.
Similarity:
Belongs to the WD repeat ESC family.
Contains 7 WD repeats.
Database links:Entrez Gene: 426381Chicken
Entrez Gene: 404183Cow
Entrez Gene: 8726Human
Entrez Gene: 13626Mouse
Entrez Gene: 398420 Xenopus laevis
Omim: 605984Human
SwissProt: Q5ZKH3Chicken
SwissProt: Q3SZ25Cow
SwissProt: O75530Human
SwissProt: Q921E6Mouse
SwissProt: Q8UUP2 Xenopus laevis
Unigene: 503510Human
Unigene: 380914Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.