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货号： bs-13033R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-13033R-PE-Cy7
- 英文名称
- Anti-Drosha/PE-Cy7
- 中文名称
- PE-Cy7标记的核糖核酸酶3/Drosha抗体
- 别 名
- DROSHA; Drosha double stranded RNA specific endoribonuclease; Drosha ribonuclease type III; Etohi2; HSA242976; Nuclear RNase III Drosha; p241; Protein Drosha; Putative protein p241 which interacts with transcription factor Sp1; Putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; Ribonuclease III nuclear; Ribonuclease type III nuclear; RibonucleaseIII; RN 3; RN3; RNase 3; RNase III; RNase3; RNASE3L; RNaseIII; RNASEN; RNC_HUMAN; RNase III Drosha.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 染色质和核信号 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 160kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Drosha/RNase III Drosha
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewings sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.
Function:
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3 and 5 strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Subunit:
Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with DGCR8, SP1 and SNIP1. Interacts with SRRT/ARS2.
Subcellular Location:
Nucleus. Nucleus; nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
Tissue Specificity:
Ubiquitous.
Similarity:
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains.
Database links:Entrez Gene: 29102 Human
Entrez Gene: 14000 Mouse
Omim: 608828 Human
SwissProt: Q9NRR4 Human
SwissProt: Q5HZJ0 Mouse
Unigene: 97997 Human
Unigene: 293142 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Dircer和Drosha是两种核糖核酸酶是卵巢癌细胞中RNA干扰机制中的关键酶，Dircer和Drosha两种酶的表达（数量）越高，存活时间越长。美国科学家研究发现，卵巢癌患者存活率与两种酶有关。美国MD安德森癌症治疗中心先后对111个和132个卵巢癌样本进行了分析。结果发现，人体内两种核糖核酸酶Dicer和Drosha含量高的患者平均存活11年，而含量低的患者平均只能活2.86年。Dicer的水平高低对卵巢癌患者尤其重要。这一发现有助于卵巢癌的诊断和治疗。卵巢恶性肿瘤的发病率在女性常见恶性肿瘤中占2.4%～5.6%，在女性生殖道恶性肿瘤中占第三位。在妇女生殖道癌瘤中，卵巢癌造成的死亡人数高居首位。