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货号： bs-12343R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-12343R-PE-Cy7
- 英文名称
- Anti-TRIB1/PE-Cy7
- 中文名称
- PE-Cy7标记的G蛋白偶联诱导蛋白2受体抗体
- 别 名
- Tribbles homolog 1; TRB-1; G-protein-coupled receptor-induced gene 2 protein; GIG-2; SKIP1; TRIB1_HUMAN; C8FW; GIG2; TRB1; Trib1; Tribbles homolog 1.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 发育生物学 信号转导 干细胞 细胞凋亡 激酶和磷酸酶 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Monkey,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 41kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from Human TRIB1/GIG2
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
TRB-1 is a 372 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase superfamily. Expressed ubiquitously with highest expression in bone marrow, thyroid gland, skeletal muscle and pancreas, TRB-1 interacts with MAPK kinases and is thought to regulate the activation of MAP kinses, possibly controlling MAP kinase cascades. The gene encoding TRB-1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.
Function:
Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.
Subcellular Location:
cytoplasm.
Tissue Specificity:
Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain.
Database links:Entrez Gene: 10221Human
Entrez Gene: 211770Mouse
Entrez Gene: 78969Rat
Entrez Gene: 521857Cow
Omim: 609461Human
SwissProt: Q96RU8Human
SwissProt: Q8K4K4Mouse
Unigene: 444947Human
Unigene: 40298Mouse
Unigene: 204391Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Trib1在脂肪组织维持和脂质代谢紊乱上发挥关键作用;
巨噬细胞至少有两个亚类，即M1型和M2型。M1型巨噬细胞参与促炎反应，且在宿主防御细菌和病毒感染中发挥核心作用。M2巨噬细胞与抗炎反应、寄生虫感染、组织重构、纤维化以及肿瘤疾病发展相关。 Trib1是个衔接蛋白，与泛素连接酶COP1相互作用参与蛋白降解。人类全基因组关联研究表明TRIB1参与脂质代谢。
日前，大阪大学WPI免疫前沿研究中心等处的研究人员，发现Trib1对F4/80 + MR +组织内在巨噬细胞的分化至关重要，且这些巨噬细胞和M2巨噬细胞、酸性粒细胞具有共同的特性(称之为M2类巨噬细胞)。但是，Trib1在M1髓系细胞就不会发挥这种作用。相关研究成果刊登在近期出版的《自然》（Nature）杂志上。
Trib1缺陷导致各种器官中M2类巨噬细胞急剧减少，如骨髓、脾脏、肺以及脂肪组织。在Trib1缺失的骨髓细胞中，C / EBP α的异常表达要为巨噬细胞分化缺陷负责。没有想到的是，造血细胞缺乏 Trib1的小鼠，减少了脂肪组织块，并伴随着脂肪分解增加，即使是在正常饮食条件下。
M2类巨噬细胞的补充可缓解这种病理学现象。这表明，这类巨噬细胞的缺乏是引发脂肪分解的原因。为了应对高脂肪饮食，造血细胞缺乏 Trib1的小鼠发育中会呈现高甘油三酯血症、胰岛素抗性，同时增加促炎细胞基因诱导。
这些结果表明Trib1在脂肪组织维持和脂质代谢紊乱上发挥关键作用，而其作用是通过控制组织内在M2类巨噬细胞的分化实现的。