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货号: bs-0287R-PE-Cy7 基本售价: 2980.0 元 规格: 100ul
产品信息
- 产品编号
- bs-0287R-PE-Cy7
- 英文名称
- Anti-His tag/PE-Cy7
- 中文名称
- PE-Cy7标记的聚组氨酸抗体标签抗体
- 别 名
- HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 标签抗体
- 研究领域
- 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 合成与降解 结合蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- fusionprotein,HHHHHHtagged
- 产品应用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- N/AkDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- Synthetic peptide: HHHHHH conjugated to KLH
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
标签抗体
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括:
细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫