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货号： bs-0924R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-0924R-PE-Cy7
- 英文名称
- Anti-Mafa/PE-Cy7
- 中文名称
- PE-Cy7标记的v-maf 肌腱膜纤维肉瘤癌基因同源物A抗体
- 别 名
- Mafa homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A;Pancreatic beta-cell-specific transcriptional activator;(avian)(V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA_MOUSE.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 神经生物学 信号转导 转录调节因子 糖尿病 内分泌病
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 37kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse Mafa
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.
Function:
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Regulates the insulin gene transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Subunit:
Binds DNA as a homodimer. Interacts with PCAF. Interacts with NEUROD1 and PDX1.
Subcellular Location:
Nucleus. Note=Detected in nuclei of pancreas islet beta cells.
Tissue Specificity:
Selectively expressed in pancreatic beta but not in alpha cells (at protein level). Expressed in eyes and at low levels in thymus. Expressed in brain, lung, spleen and kidney. Expressed in embryo.
Post-translational modifications:
Ubiquitinated, leading to its degradation by the proteasome.
Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable).
Similarity:
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
Database links:Entrez Gene: 389692 Human
Entrez Gene: 378435 Mouse
Entrez Gene: 366949 Rat
Omim: 610303 Human
SwissProt: Q8NHW3 Human
SwissProt: Q8CF90 Mouse
Unigene: 521914 Human
Unigene: 309589 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.