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货号： bs-7007R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-7007R
- 英文名称
- RNF43
- 中文名称
- 环指蛋白43抗体
- 别 名
- RNF124; Ring finger protein 43; URCC; RNF43_HUMAN; E3 ubiquitin-protein ligase RNF43; RING finger protein 43.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 肿瘤细胞生物标志物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 83kDa
- 细胞定位
- 细胞核 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human RNF43:41-140/783
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Function:
Probable E3 ubiquitin-protein ligase. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Subunit:
Interacts with AKAP8L, NONO and SFPQ.
Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Nucleus envelope.
Tissue Specificity:
Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.
Similarity:
Contains 1 RING-type zinc finger.
SWISS:
Q68DV7
Gene ID:
54894
Database links:Entrez Gene: 54894 Human
Entrez Gene: 207742 Mouse
Entrez Gene: 303412 Rat
Omim: 612482 Human
SwissProt: Q68DV7 Human
SwissProt: Q5NCP0 Mouse
Unigene: 584916 Human
Unigene: 440230 Mouse
Unigene: 214174 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-RNF43 (bs-7007R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 93 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RNF43 Polyclonal Antibody, Unconjugated(bs-7007R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining