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货号： bs-1721R-PE-Cy7 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-1721R-PE-Cy7
- 英文名称
- Anti-dUTPase/PE-Cy7
- 中文名称
- PE-Cy7标记的脱氧尿嘧啶三磷酸核苷水解酶抗体
- 别 名
- Deoxyuridine 5 triphosphate nucleotidohydrolase, nuclear isoform; dUTP pyrophosphatase; dUTPase; FLJ20622; Deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial; Deoxyuridine triphosphatase; dut; DUT_HUMAN; dUTP nucleotidohydrolase.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 20kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human dUTPase (180-124aa)
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008].
Function:
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Subunit:
Homotrimer.
Subcellular Location:
Isoform 2: Nucleus. Isoform 3: Mitochondrion.
Tissue Specificity:
Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).
Post-translational modifications:
Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
The initiator methionine is cleaved in isoform 2.
Similarity:
Belongs to the dUTPase family.
Database links:Entrez Gene: 1854 Human
Entrez Gene: 110074 Mouse
Entrez Gene: 497778 Rat
Omim: 601266 Human
SwissProt: P33316 Human
SwissProt: P70583 Rat
Unigene: 527980 Human
Unigene: 282499 Mouse
Unigene: 6102 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
dUTPase是机体中重要的水解酶，基本作用是把dUTP水解成dUMP和焦磷酸，不但可以提供给DNA的合成原料，还可以降低DNA突变的几率，对细胞遗传起到一定的稳定作用。