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货号： bs-6592R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-6592R
- 英文名称
- HSD17B6
- 中文名称
- 17-β-羟脱氢酶6抗体
- 别 名
- 17-beta-HSD 6; 17-beta-HSD6; 17-beta-hydroxysteroid dehydrogenase type 6; 3 hydroxysteroid epimerase; 3(alpha >beta) hydroxysteroid epimerase; 3(alpha >beta) hydroxysteroid epimerasel; 3-alpha->beta-HSE; 3-alpha->beta-hydroxysteroid epimerase; H17B6_HUMAN; HSD17B6; HSE; Hydroxysteroid (17 beta) dehydrogenase 6; Hydroxysteroid (17 beta) dehydrogenase 6 homolog (mouse); Hydroxysteroid 17 beta dehydrogenase 6; NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase; NAD+ dependent 3 alpha hydroxysteroid dehydrogenase; Oxidative 3 alpha hydroxysteroid dehydrogenase; Oxidative 3-alpha hydroxysteroid dehydrogenase; Oxidoreductase; Retinol dehydrogenase; RODH; SDR9C6;Short chain dehydrogenase/reductase family 9C, member 6.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 信号转导 生长因子和激素
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 33kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human HSD17B6:61-160/317
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
Tissue specificity; Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
Sequence similarities; Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Subcellular Location:
Microsome membrane; Peripheral membrane protein; Lumenal side. Early endosome membrane; Peripheral membrane protein; Lumenal side (Potential).
Tissue Specificity:
Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
Similarity:
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
SWISS:
O14756
Gene ID:
8630
Database links:Entrez Gene: 8630Human
Entrez Gene: 27400Mouse
Entrez Gene: 286964Rat
Omim: 606623Human
SwissProt: Q3T001Cow
SwissProt: O14756Human
SwissProt: Q9R092Mouse
SwissProt: O54753Rat
Unigene: 524513Human
Unigene: 26719Mouse
Unigene: 10857Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSD17B6 Antibody(bs-6592R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-HSD17B6 antibody (bs-6592R)
Dilution:3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.